I. Unit Cell Processes and Mechanics of Matrix Molecules and Tissues Structures

1

Clinical Examples of the Roles of Mechanical Forces in Tissues and Organs: The Working Paradigms

Prof. Spector

(PDF)

2

Tissue Structures and Unit Cell Processes

Prof. Spector

(PDF 1)

(PDF 2)

(PDF 3)

3

Cell-Matrix Interactions: Extracellular Matrix Molecules, Adhesion Proteins and Integrins

Prof. Spector

(PDF 1)

(PDF 2)

4

Models for the Mechanical Behavior of Porous Scaffolds

Prof. L. Gibson

(PDF)

5

Structure-Properties Relationships for Tissues

Prof. Spector

6

Mechanics of Selected Tissues

Prof. S. Socrate

II. Mechanics of Cells: Effects of Exogenous Forces and Endogenous Force Generation

7

Effects of Exogenous Mechanical Forces on Cells

Prof. Spector

8

Response of Cells to Substrate Strain

Prof. Spector

(PDF)

9

Endothelial Cell Response to Flow

Prof. C. F. Dewey

10

Quiz 1

11

Measuring Cell Contraction

Cell Force Monitor

Brendan Harley

(PDF)

12

Endogenous Mechanical Force Generation by Cells

Prof. Spector

(PDF 1)

(PDF 2)

13

Models for Cell Contraction In Vitro and In Vivo

Prof. Spector

(PDF 1)

(PDF 2)

14

Mechanical Coupling of Cells with Matrix

Prof. Yannas

(PDF 1)

(PDF 2)

15

Cell-matrix Interactions During Wound Closure

Prof. Yannas

(PDF - 1.6 MB)

16

Blockade of Contraction During Induced Organ Regeneration

Prof. Yannas

(PDF - 1.6 MB)

17

Review

Prof. Spector

18

Quiz 2

III. Tissue Mechanics

19

Review of Principles of Linear Elastic Mechanics

Prof. Yannas

(PDF - 1.1 MB)

20

Nonlinear Elasticity: Tendon and Skin

Prof. Yannas

(PDF)

21

Linear Viscoelastic Behavior

Prof. Yannas

(PDF - 1.0 MB)

22

Response of Articular Cartilage to Mechanical Loading

Prof. A. Grodzinsky

23

Mechanical Behavior of Ligament, Meniscus and Intervertebral Disc

Prof. Spector

(PDF 1)

(PDF 2)

24

Mechanical Behavior of Bone

Prof. Spector

(PDF)

25

Response of Bone to Mechanical Loading

Review

Prof. Spector

(PDF)

26

Quiz 3

Homework 1 (PDF)

(PDF)

Homework 2 (PDF)

(PDF)

Homework 3 (PDF)

I. Unit Cell Processes and Mechanics of Matrix Molecules and Tissues Structures

1

Clinical Examples of the Roles of Mechanical Forces in Tissues and Organs: The Working Paradigms

Spector notes (PDF

2

Tissue Structures and Unit Cell Processes

Spector notes (PDF)

3

Cell-Matrix Interactions: Extracellular Matrix Molecules, Adhesion Proteins and Integrins

Spector notes (PDF 1) (PDF 2)

Stossel, Thomas P. "On the Crawling of Animal Cells." Science, New Series, 260, no. 5111 (May 21, 1993): 1086-1094.

Hinz, Boris, Giuseppe Celetta, James J. Tomasek, Giulio Gabbiani, and Christine Chaponnier. "Alpha-Smooth Muscle Actin Expression Upregulates Fibroblast Contractile Activity." Molecular Biology of the Cell 12 (September 2001): 2730-2741.

Vale, Ronald D., and Ronald A. Milligan. "The Way Things Move: Looking Under the Hood of Molecular Motor Proteins." Science 288 (April 7, 2000).

4

Models for the Mechanical Behavior of Porous Scaffolds

5

Structure-Properties Relationships for Tissues

6

Mechanics of Selected Tissues

II. Mechanics of Cells: Effects of Exogenous Forces and Endogenous Force Generation

7

Effects of Exogenous Mechanical Forces on Cells

Spector notes (PDF)

8

Response of Cells to Substrate Strain

Banes, Albert J., Mari Tsuzaki, Juro Yamamoto, Thomas Fischer, Brian Brigman, Thomas Brown, and Larry Miller. "Mechanoreception at the Cellular Level: The Detection, Interpretation, and Diversity of Responses to Mechanical Signals."Biochem Cell Biol 73 (1995): 349-365.

Davies, Peter F., and Satish C. Tripathi. "Mechanical Stress Mechanisms and the Cell - An Endothelial Paradigm." Circulation Research 72, no. 2 (February, 1993): 239-245.

Wang, Ning, James P. Butler, and Donald E. Ingber. "Mechanotransduction Across the Cell Surface and Through the Cytoskeleton." Science, New Series, 260, no. 5111 (May 21, 1993): 1124-1127.

Alenghat, Francis J., and Donald E. Ingber. "Mechanotransduction: All Signals Point to Cytoskeleton, Matrix, and Integrins." Sciences, STKE (Feb 12, 2002).

Schaffer, Jonathan L., Michael Rizen, Gilbert J. L'Italien, Aziz Benbrahim, Joseph Megerman, Louis C. Gerstenfeld, and Martha L. Gray. "Device for the Application of a Dynamic Biaxially Uniform and Isotropic Strain to a Flexible Cell Culture Membrane." Journal of OrthoPaedic Research 12: 709-719.

McNeil, Paul L., and Richard A. Steinhardt. "Loss, Restoration, and Maintenance of Plasma Membrane Integrity." The Journal of Cell Biology 137, no. 1 (April 7, 1997): 1-4.

McNeil, Paul L., and Richard A. Steinhardt. "Plasma Membrane Disruption: Repair, Prevention, Adaptation." Annu Rev. Cell Dev. Biol. 19 (July 8, 2003): 697-731.

Miyake, Katsuya, and Paul L. McNeil. "Mechanical Injury and Repair of Cells." Crit Care Med 31, no. 8 (Suppl. 2003).

9

Endothelial Cell Response to Flow

10

Quiz 1

11

Measuring Cell Contraction

Cell Force Monitor

12

Endogenous Mechanical Force Generation by Cells

13

Models for Cell Contraction In Vitro andIn Vivo

14

Mechanical Coupling of Cells with Matrix

Yannas, Chapter 8.

15

Cell-matrix Interactions During Wound Closure

Yannas, Chapter 9.

16

Blockade of Contraction During Induced Organ Regeneration

Yannas, Chapter 9.

17

Review

Oliver, Tim, Juliet Lee, and Ken Jacobson. "Forces Exerted by Locomoting Cells."Cell Biology 5 (1994): 139-147.

Rutherford, Dr. Jack. "Structure and Function of Skeletal Muscle."

Wu, Richard C. "Physiology of smooth muscle."

18

Quiz 2

III. Tissue Mechanics

19

Review of Principles of Linear Elastic Mechanics

Yannas, Chapter 10.

20

Nonlinear Elasticity: Tendon and Skin

Yannas, Chapter 11.

21

Linear Viscoelastic Behavior

Yannas, Chapter 9.

22

Response of Articular Cartilage to Mechanical Loading

23

Mechanical Behavior of Ligament, Meniscus and Intervertebral Disc

24

Mechanical Behavior of Bone

25

Response of Bone to Mechanical Loading

Review

26

Quiz 3